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2-amino-4-phosoponobutyrate. When selectively applied to the mud puppy retina the On-bipolars, On-amicrines and On-ganglion cells become unresponsive to light, whereas the Off-cells retain their response properties. Allows for the study of the Off-cell pathway in isolation. Major finding is that the center-surround antagonism of Off-center retinal ganglion cells is not significantly altered suggesting that it is not a product of the interaction between On and Off cells.