See Also: immunoblot(medicine)
Kim Young Sam(encyclopedia)
young(medicine)
Young, Cy(dictionary)
young(1)(dictionary)
young(2)(dictionary)
Young, Cy(encyclopedia)
Young, Neil(encyclopedia)
Young, Coleman(encyclopedia)
Young, Thomas(encyclopedia)

Young-Helmholtz (iou) and immunoblot (medicine)


Young-Helmholtz (iou)



Young-Helmholtz noun. L19.
[from Thomas Young (see YOUNG'S MODULUS) + H. L. F. von HELMHOLTZ.]
Used attrib. to designate the theory that in the eye there are receptors sensitive to one of three colours (red, green, and violet), and that colour perception is due to the stimulation of these in different proportions.

immunoblot (medicine)


immunoblot


Commonly referred to as Western blot because of the similarity to the procedures described above, is used to detect antibodies to specific epitopes of electrophoretically separated subspecies of antigens. Electrophoresis of antigenic material yields separation of the antigenic components by molecular weight. Blotting of the separated antigen to nitrocellulose, retaining the electrophoretic position, and reacting it with patient specimen will result in the binding of specific antibodies, if present, to each antigenic "band". Electrophoresis of known molecular weight standards allows for the determination of the molecular weight of each antigenic band to which antibodies may be produced. These antibodies are then detected using EIA reactions which characterise antibody specificity. This technique is often used to confirm the specificity of antibodies which are detected by ELISA screening procedures.