See Also: Southern blot(medicine)
Blot, Southern(health)
blot, southern(medicine)
Southern blot(dictionary)
Southern blot analysis(medicine)
Herbal medicine (botanical medicine, herbology, phytomedicine)(health)
blot(dictionary)
blot(medicine)
blot(1)(dictionary)
Dot blot(medicine)

Southern blot (medicine)


southern blot


A technique used for searching for a specific DNA fragment. The process is as follows:

1. separate DNA fragments by gel electrophoresis

2. Change pH of gel to basic, thus allowing disruption of H-bonds

3. blot gel with nitrocellulose paper

4. Heat paper so as to fix DNA fragments

5. probe with labelled messenger RNA or cDNA

6. washComplementary mRNA/cDNA fragments will have hybridised.