See Also: bandshift assay(medicine)
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assay(1)(dictionary)

bandshift assay (medicine)


bandshift assay
<investigation> An assay for proteins, such as transcription factors, that bind specific DNA sequences.

A labelled oligonucleotide corresponding to the recognition sequence is incubated with an appropriate nuclear protein extract and run on a nondenaturing acrylamide gel. Oligonucleotides that have been bound by proteins are retarded relative to those that are unbound.