See Also: DNA footprinting(medicine)
footprinting(medicine)
DNAse footprinting(medicine)
Herbal medicine (botanical medicine, herbology, phytomedicine)(health)
Medicine Lodge Memorial Hospital- Medicine Lodge(health)
Orthomolecular medicine (orthomolecular nutritional medicine, orthomolecular therapy)(health)
medicine(encyclopedia)
medicine man(encyclopedia)
Medicine(medicine)
medicine(dictionary)

footprinting (medicine)


footprinting
<molecular biology, technique> A technique used to identify the binding site of, for example: a protein on a nucleic acid sequence.

The basic principle is to carry out a very limited hydrolysis of the DNA with or without the protein complexed and then to compare the digestion products. If a cleavage site is masked by the bound protein then the pattern of fragments when protein is present will be different and it is possible to work out, by a series of such procedures, exactly where the protein binds.